"Uni-Polar Clustering of Lymphocyte DNA Templates Toward Neoplastic Target Cells Within Hodgkin’s Disease Lymph Nodes".
John Frenster, Physicians’ Educational Series, Atherton, CA 94027-5446.
E-mail: frenster@euchromatin.net
Five hundred and two cells, selected by serial focusing on at least
one different neoplastic cell within the lymphoma-involved lymph nodes
of pre-treatment patients with the nodular sclerosis type of Hodgkin's
Disease, were analyzed by electron microscopy for in-vivo intracellular
activity and intercellular interaction (J. Natl. Cancer
Inst., 63: 331 (1979). Neoplastic cells (no. = 31) represented 5% of
the total sampled cell population, whereas lymphocytes (no. = 437) represented
87% of the sampled population. Activation of DNA templates within neoplastic
Reed-Sternberg and Hodgkin’s cells and within apposed lymphocytes was recognized
by ultrastructural DNase I-sensitive probes (Cancer
Res., 31: 1128 (1971). Active lymphocytes were most often found apposed
to the more active of the neoplastic cells, and the nuclei of such apposed
lymphocytes displayed a progressive preferential uni-polar clustering of
active DNA templates into that half of the lymphocyte nucleus closest to
the neoplastic cell. Such uni-polar clustering of active DNA templates
may reflect spatially-directed intracellular responses to intercellular
contacts between activated lymphocytes and their neoplastic cell targets.
Supported in part by USPHS Research Grants CA-10174 and CA-13524
from the National Cancer Institute, by Research Grant IC-45 from the American
Cancer Society, and by a Research Scholar Award from the Leukemia
Society of America.
1. Maruo S, Nanbo A, and Takada K, "Replacement of the Epstein-Barr Virus Plasmid with the EBER Plasmid in Burkitt's Lymphoma Cells".
2. Frenster JH, Papalian MM, Masek MA, and Frenster JA, "Electron Microscopic Analysis of Lymph Node Cellular Activity in Hodgkin's Disease".
3. Frenster JH, "Electron Microscopic Localization of Acridine Orange Binding to DNA within Human Leukemic Bone Marrow Cells".
4. Frenster JH, "Ultrastructural Probes of Active DNA Sites, and the RNA Activators of DNA".
5. Frenster JH, "Activation of DNA Transcription within Repressed Chromatin".
6. Carella AM, "Stem Cell Transplantation for Hodgkin's Disease".
7. Faulkner RD, Craddock C, Byrne JL, Mahendra P, Haynes AP, Prentice HG, Potter M, Pagliuca A, Ho A, Devereux S, McQuaker G, Mufti G, Yin JL, and Russell NH, "BEAM-Campath reduced intensity allogeneic stem cell transplantation for lymphoproliferative disease: GVHD, toxicity and survival in 65 patients".
8. Masek MA, Rhoades DJ, and Frenster JH, "In-Vivo Macrophage Interactions with Lymphocytes in Hodgkin's Disease", Proc. Am. Assoc. Cancer Res. 14: 8 (1973).
9. Rowan RA, Masek MA, Thompson JM, and Frenster JH, "Electron Microscopic Localization of Acid Phosphatase Activity within Hodgkin's Disease Lymph Nodes", Proc. Am. Assoc. Cancer Res. 16: 10 (1975).
10. Chen JJW, Lin Y-C, Yao P-L, Yuan A, Chen H-Y, Shun C-T, Tsai M-F, Chen C-H, Yang P-C, "Tumor-Associated Macrophages: The Double-Edged Sword in Cancer Progression", Journal of Clinical Oncology, vol. 23, no. 5, pp. 953-964 (February 10, 2005).
Top of Page - Euchromatin Network - Current Research - Forums - Other Sites - Future Events -