•1. DNase
I-sensitive probes allow identification of active DNA sites within intact cells.
•
•2. Such sites vary
characteristically during cell activation, cell division, and cell differentiation.
•
•3. During
lymphocyte activation within Hodgkin lymphoma lymph nodes, active DNA sites undergo unipolar clustering toward
neoplastic cells.
•
•4. Such unipolar
clustering may reflect signals from the neoplastic cells to the surrounding lymphocytes.
•
•5. DNase I-sensitive probes
permit the analysis of euchromatin activation
• by RNA within human intact cells. ( www.euchromatin.net
).