Presented at the 42nd Annual Meeting of the American Society for Cell Biology, Moscone Convention Center, San Francisco, California, USA,  December 18, 2002, as Late Poster Abstract L235.
“A Specific Actin-Ribonucleoprotein Interaction Required for Transcription by RNA Polymerase II”,

P. Percipalle 1, N. Fomproix 1, K. Kylberg 1, F. Miralles 2, B. Bjorkroth 1, B. Daneholt 1, and N. Visa 2,

1 Karolinska Institute, Stockholm, Sweden and 2 Stockholm University, Stockholm, Sweden.

E-mail:  Neus.Visa@molbio.su.se



Abstract:

Actin is present not only in the cytoplasm but also in the cell nucleus, where it has been implicated in transcription of protein-coding genes and nuclear export. We have previously shown that actin is a bona fide component of the Balbiani ring pre-messenger ribonucleoprotein particles (BR pre-mRNPs) in the salivary gland cells of the dipteran Chironomus tentans, and we have shown that actin binds directly to the hnRNP protein hrp36. We recently discovered another nuclear actin-binding protein, hrp65-2. Hrp65-2 belongs to a family of evolutionarily conserved proteins with multiple roles in gene expression. We have mapped the actin-binding domain of hrp65-2 to the C-terminal sequence of the protein, and we have shown that a synthetic peptide corresponding to the C-terminus of hrp65-2 binds directly to actin in vitro, disrupts the actin-hrp65-2 interaction both in vivo and in vitro, and causes a global transcription inhibition in living cells. Our results raise the possibility that an actin-based mechanism is implicated in the transcription of most, if not all, RNA polymerase II genes, and they suggest that an actin-hrp65-2 interaction is required to maintain the normal transcriptional activity of the cell. Furthermore, both actin and hrp65-2 are associated with the nascent BR pre-RNA along the transcribed BR gene, which is consistent with the idea that an actin-hrp65-2 complex plays a role during transcription elongation.


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30. Frenster JH, and Hovsepian JA, "RNA Feedback Mechanisms during Eukaryotic Gene Regulation".

31. Frenster JH, "Ultrastructural Probes of Active DNA Sites, and the RNA Activators of DNA".
 



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