"Sequential Entry of Components of Gene Expression Machinery into Daughter Nuclei".
Kannanganattu V. Prasanth, Paula A. Sacco-Bubulya, Supriya G. Prasanth, and David L. Spector*
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724
* Corresponding author. E-mail address: spector@cshl.org
In eukaryotic cells, RNA polymerase II (RNA pol II) transcription
and pre-mRNA processing are coordinated
events. We have addressed how individual components of the transcription
and pre-mRNA processing
machinery are organized during mitosis and subsequently recruited
into the newly formed daughter nuclei.
Interestingly, localization studies of numerous RNA pol II transcription
and pre-mRNA processing factors
revealed a nonrandom and sequential entry of these factors into
daughter nuclei after nuclear envelope/lamina
formation. The initiation competent form of RNA pol II and general
transcription factors appeared in the
daughter nuclei simultaneously, but prior to pre-mRNA processing
factors, whereas the elongation competent
form of RNA pol II was detected even later. The differential entry
of these factors rules out the possibility that
they are transported as a unitary complex. Telophase nuclei were
competent for transcription and pre-mRNA
splicing concomitant with the initial entry of the respective factors.
In addition, our results revealed a low
turnover rate of transcription and pre-mRNA splicing factors during
mitosis. We provide evidence to support a
model in which the entry of the RNA pol II gene expression machinery
into newly forming daughter nuclei is a
staged and ordered process.
Additional References:
#A. Frenster JH, and Hovsepian JA, "Overshoot in Late Telophase for RNA Re-Programming of Mitotic Chromatin","RNA2003", p. 211 (July 1-6, 2003), The RNA Society, Bethesda, MD, USA.
1. Nakatsu SL, Masek MA, Landrum S, and Frenster JH, "Activity of DNA Templates During Cell Division and Cell Differentiation", Nature 248: no. 5446, pp. 334-335 (March 22, 1974).
2. Goldstein L, “Stable Nuclear RNA Returns to Post-Division Nuclei Following Release to Cytoplasm during Mitosis”, Exp. Cell Res. 89: 421-425 (1974).
3. Saha S , Ansari AZ, Jarell KA, and Ptashne M, "RNA Sequences that Work as Transcriptional Activating Regions".
4. Frenster JH, “Nuclear Polyanions as De-Repressors of Synthesis of Ribonucleic Acid”.
5. Frenster JH, "Nuclear RNA Species Activate DNA Transcription Within Chromatin".
6. Hovsepian JA, and Frenster JH, "RNA-Induced Melting of DNA during Selective Gene Transcription".
7. Frenster JH, "Ultrastructural Probes of Active
DNA Sites, and the RNA Activators of DNA".