Fig.
3 (left) and Fig. 4 (right).Isolated lymphocyte nuclei after extraction of nuclear ribosomes. Masses of condensed (hetero-)chromatin, fibrils of more extended (eu-)chromatin, and an occasional light-staining nucleolus.
Published in: Proceedings of the National
Academy of Sciences, U.S., vol. 50, no. 6, pp. 1028-9 (Dec. 1963):
Electron Micrographs for:
"Repressed and Active Chromatin Isolated from Interphase Lymphocytes".
John H. Frenster, Vincent G. Allfrey, and Alfred E. Mirsky
Rockefeller Institute, New York, N.Y. 10021
Fig.
1 (left) and Fig. 2 (right).Fig.
3 (left) and Fig. 4 (right).
Isolated lymphocyte nuclei after extraction of nuclear ribosomes. Masses
of condensed (hetero-)chromatin, fibrils of more extended (eu-)chromatin,
and an occasional light-staining nucleolus.
Fig.
5 (left) and Fig. 6 (right).
Swollen nuclei in cation-free isotonic sucrose. Extended (eu-)chromatin
fibrils are seen attached to the condensed (hetero-)chromatin masses, with
fibril dispersion distally.
Fig.
7 (left) and Fig. 8 (right).
Isolated condensed (hetero-)chromatin masses. A small number of euchromatic
fragments remain attached to the masses.
Fig.
9 (left) and Fig. 10 (right).
Fig. 9. Isolated extended (eu-)chromatin fibrils. No condensed (hetero-)chromatin
masses are found.
Fig. 10. Particles in 10 mM calcium. Aggregates of dense particles are
evident, resembling those in isolated nucleoli.
Additional References:
0. Electron Microscopy of Human Lymphocytes before and after Activation
by PHA (Busch H, 1974).
Top of Page - Return to
Main Body of Paper - Euchromatin Network Home
Page - Current Research - Links
-
For Further Information and Feedback:
E-mail: frensasc@ix.netcom.com
John H. Frenster, M.D.
Physicians' Educational Series
247 Stockbridge Avenue
Atherton, CA 94027-5446
Phone: 650/367-6483
Fax: 650/364-1773
WebSite: http://www.frenster.com
euchromatin: "the most active portion of the genome within the
cell nucleus".