Richard A. Lease, Michael E. Cusick, and Marlene Belfort.
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, P.O. Box 22002, Albany, New York 12201-2002.
Edited by Sidney Altman, Yale University, New Haven, CT, and approved July 16, 1998 (received for review May 18, 1998).
DsrA is an 87-nt untranslated RNA that regulates both the global
transcriptional silencer and nucleoid protein H-NS and the stationary
phase and stress response sigma factor RpoS. We demonstrate that DsrA acts
via specific RNA:RNA base pairing interactions at the hns
locus to antagonize H-NS translation. We also give evidence
that supports a role for RNA:RNA interactions at the rpoS
locus to enhance RpoS translation. Negative regulation of hns
by DsrA is achieved by the RNA:RNA interaction blocking translation
of hns RNA. In contrast, results suggest that positive regulation
of rpoS by DsrA occurs by formation of an RNA structure that
activates a cis-acting translational operator. Sequences within
DsrA complementary to three additional genes, argR, ilvIH,and
rbsD,
suggest that DsrA is a riboregulator of gene expression that acts coordinately
via RNA:RNA interactions at multiple loci.
Additional References:
1. "Switching On and Off with RNA".
3. "The Role of Chromosomal RNAs in Marking the X for Dosage Compensation".
4. "Oncogenes as Molecular Targets within Active Chromatin".
5. "Mated Models of Gene Regulation in Eukaryotes".
6. "Nuclear Polyanions as De-Repressors
of Synthesis of Ribonucleic Acid".
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