Published in: "Advances in Cell and Molecular Biology", vol. 3, pp. 1-19, (1974), ed. DuPraw EJ, New York: Academic Press.

"Ultrastructural Probes of DNA Templates within Human Bone Marrow and Lymph Node Cells."

John H. Frenster, Shirley L. Nakatsu, and Marilyn A. Masek
Division of Medical Oncology, Department of Medicine
Stanford University School of Medicine, Stanford, California 94305

Fig. 1. Probe analysis of DNA templates within differentiating granulocytes in normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromtin portion of the cell nucleus.
(A, left): Promyelocyte. A nucleolus is present in the nucleus. Three types of immature granules are seen in the cytoplasm, together with a centriole, a well-developed Golgi apparatus, and a significant amount of endoplasmic reticulum. X6,500. (B, right): Myelocyte. Only two types of granules are seen in the cytoplasm. X6,500.

Fig. 2. Probe analysis of DNA templates within differentiating erythrocytes in normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus.
(A, left): Proerythroblast. The cytoplasm contains numerous polyribosomes and fine ferritin granules, with evidence of rhopheocytosis at the plasma membrane. X6,500. (B, right): Erythroblast. The cytoplasm contains numerous polyribosomes, but lesser degrees of ferritin or rhopheocytosis. X6,500.

Fig. 3. Probe analysis of DNA templates within differentiating monocytes in normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus.
(A, left): Early monocyte. The cytoplasm contains a centriole, a well-developed Golgi apparatus, and a small number of dense granules. X6,500. (B, right): Late monocyte. The cytoplasm contains short segments of rough endoplasmic reticulumand an increasing number of dense granules. X6,500.

Fig. 4. Probe analysis of DNA templates within mononuclear cells in normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus. (A, left): Macrophage. The plasma membrane is highly convoluted, and the cytoplasm contains numerous short segments of rough endoplasmic reticulum. X 3,750. (B, right): Undifferentiated cell (? stem cell).The cytoplasm is extremely scanty and contains only an occasional mitochondrion. X7,500.

Fig. 5. Probe analysis of DNA templates within lymphoid cells of normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus. (A, left): Small lymphocyte. The cytoplasm contains monoribosomes but no dense granules or endoplasmic reticulum. X13,000.
(B, right): Plasma cell. The cytoplasm contains a large amount of rough endoplasmic reticulum, a homogeneous inclusion body, and a large Golgi area. X7,500.

Fig. 6. Probe analysis of DNA templates within reticuloendothelial cells of normal human bone marrow. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus.
(A, left): Endothelial cell. The endothelial cell is lining a sinus (above) and displays an active plasma membrane toward the surrounding marrow cord (below). x3,250. (B, right): Reticulum cell. The cytoplasm contains numerous phagosomes and ferritin particles, and the plasma membrane is extremely convoluted. X3,250.