Published in: "Advances in Cell and Molecular Biology", vol. 3, pp. 1-19, (1974), ed. DuPraw EJ, New York: Academic Press.

"Ultrastructural Probes of DNA Templates within Human Bone Marrow and Lymph Node Cells."

John H. Frenster, Shirley L. Nakatsu, and Marilyn A. Masek
Division of Medical Oncology, Department of Medicine
Stanford University School of Medicine, Stanford, California 94305

(Electron Micrographs for: "Ultrastructure and Function of Heterochromatin and Euchromatin".)

(Electron Micrographs of In-Vivo Neoplastic Cells in Hodgkin's Disease Lymph Nodes.)

Fig. 7. Probe analysis of DNA templates within lymphocytes of a positive lymph node taken from an untreated patient with Hodgkin's disease, nodular sclerosis type. Electron-dense reaction products are localized exclusively within the euchromatin portion of the cell nucleus. (A, left): Monoribosomal lymphocyte. The cytoplasm contains a centriole, and the ribosomes are largely arrayed in a monosomal manner. X13,000. (B, right): Polyribosomal lymphocyte. The cytoplasmic ribosomes are largely arrayed in a polysomal manner. X9,750.

Fig. 8. Probe analysis of DNA templates within polysomal cells of a positive lymph node taken from an untreated patient with Hodgkin's disease, nodular sclerosis type. (A, left): Large polysomal cell with electron-dense reaction products localized exclusively within the euchromatin portion of the cell nucleus. Aside from clusters of polyribosomes, the cytoplasm contains a rare strand of rough endoplasmic reticulum and small numbers of dense granules near the Golgi apparatus. X6,500. (B, right): Large polysomal cell in early telophase of mitosis, with condensed chromosomes, centriole with mitotic spindle, and early reconstitution of the nuclear membrane. The electron-dense reaction products are localized to the euchromatin portion of the cell nucleus between the masses of condensed chromosomes. The cytoplasm contains clusters of polyribosomes and a rare strand of rough endoplasmic reticulum. X6,500.

Fig. 9. Probe analysis of DNA templates within neoplastic cells of a positive lymph node taken from a patient with Hodgkin's disease, nodular sclerosis type. (A, left): Large mononuclear Reed-Sternberg cell, with electron-dense reaction products localized exclusively within the euchromatin portion of the cell nucleus. The nucleus is irregularly shaped, invaginated, and contains a large nucleolus, while the cytoplasm is largely polyribosomal with an occasional strand of rough endoplasmic reticulum. X7,500. (B, right): Polynuclear Reed-Sternberg cell, showing almost no reaction products, while surrounding lymphocytes are heavily labeled by reaction products. Each of the lobes of the nucleus are themselves invaginated and at least three large nucleoli are evident. The cytoplasm is polyribosomal with an occasional thin strand of rough endoplasmic reticulum. X3,750.

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